EFFECT OF GRADUAL PERFUSION BY MODERATE HYPOTHERMIA FOR AVOIDING ISCHEMIC- REPERFUSION DAMAGE OF RENAL ALLOGRAFT

Abstract

We aimed to define the effects of vasoconstriction related to hypothermia during flushout perfusion on renal tissue micro sir culation in repeifusion period. 

In rabbit's model in the 1st group explanted kidneys were perfused with (UW) solution at 4 C for 20 minutes, in the 2nd group perfusion was performed with UW solution at 22 C for 10 min., then at 4 C for the next 10 min. All kidneys were reperfused with the autologous blood after 48 and 96 hours' preservation.

Median perfusate flow was significantly higher in the 2nd group in contrast to 1st one during perfusion and reperfusion. On electron microscopic examinations in the 1st group erythrocyte's aggregation in arterioles and capillaries, and after 48 hours' preservation erythrocyte agglutination and adhesion to the endothelium were observed. Endothelial defects and erythrocyte extravasation were seen following reperfusion. In the 2nd group arterioles and capillaries were free of blood elements and microcirculation was better in reperfusion period. In the 1st group lipid peroxidation levels in renal tissue was higher and glutathion level was lower in comparison to the 2nd group.

We concluded that gradual perfusion decreases the iscemic-reperfusion damages on renal allograft.